Experimental and Computational Micro-Characterization Techniques in Wood Mechanics

Curriculum Vitae


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Research Projects (relevant to Action)

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Domestic projects

“Cellular response to pollution stress in trees. Possibility of application in environmental monitoring” 2001-2005, financed by the Ministry of science of Rep Serbia- project leadership

“Investigations of novel biological sensors for plant monitoring and diagnostics” 2006-2010, financed by the Ministry of science of Rep Serbia- project leadership

- We are studying structure of the cell walls and constituent polymers, as well as antioxidant enzymes (specially those responsible for synthesis of polyphenolic compounds in the cell walls) and simple phenolic compounds, in order to find natural indicators that may be used in monitoring of the early stress in trees. Another direction of studies is synthesis of nanoparticles based on semiconductors (CdSe), and their linking to the chosen indicators of stress (such as proteins or polyphenols).

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International projects

“Infrared microspectrometry of the plant cell walls” 2001, financed by LURE (Laboratoire pour l’utilisation du rayonnement electromagnetique, Orsay, France)

- We tried to map differences in the FTIR spectra of the tissue sections between stressed and unstressed tree

“Fluorescence lifetime and spectra of lignin and related compounds” 2001, financed by LURE (Laboratoire pour l’utilisation du rayonnement electromagnetique, Orsay, France)

- We measured fluorescence decay associated emission and excitation spectra of the synthesized lignin model compound, to detect molecular emitters (fluorophores) within the lignin polymer, which is important in its structural studies

“New methods for the investigation of the structure of plant cell walls” 2004-2005, bilateral project with Laboratory for cellular biology, Versay, France, financed by Ministry of science of Rep Serbia and Ministry of foreign affaires of France (through the organisation Egide)

- We studied differences in FTIR spectra of the Arabidopsis stem sections between wild type plants and mutants in the pathway of lignin biosynthesis

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Selected Publications (relevant to Action)

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Radotić K., Simić-Krstić J., Jeremić M., Trifunović M. (1994): "Study of lignin formation at the molecular level by scanning tunneling microscopy". - Biophysical Journal 66, 1763-1767

Radotić K., Todorović S., Zakrzewska J., Jeremić M.: (1998): Study of photochemical reactions of coniferyl alcohol. II. Comparative structural study of a photochemical and enzymatic polymer of coniferyl alcohol. - Photochemistry and Photobiology 68, 703-709

Mićić M., Orbulescu J., Radotić K., Jeremić M., Sui G., Zheng Yu., Leblanc R.M. (2002): “ZL- DHP lignin model compound at the air–water interface”. – Biophys. Chem. 99, 55–62

Mićić M., Radotić K., Jeremić M., Leblanc R. (2003): “Study of self-assembly of the lignin model compound on cellulose model substrate”. – Macromolecular Bioscience 3, 100-106

Mićić M., Radotić K., Jeremić M., Djikanović D., Kämmer S. (2004): “Study of lignin model compound supramolecular structure by combination of near-field scanning optical microscopy and atomic force micreoscopy”. – Colloids and Surfaces B: Biointerfaces 34, 33-40

Radotić K., Mićić M., Jeremić M. (2005): “New insights into the structural organization of the plant polymer lignin”. - Ann. N.Y. Acad. Sci. 1048, 215–229

Radotić K., Kalauzi A., Djikanović D., Jeremić M., Leblanc R. M., Cerović Z. G. (2006): “Component analysis of the fluorescence spectra of a lignin model compound”. – J. Photochem. Photobiol. B: Biology 83, 1-10

Radotić K., Mićić M., Jeremić M. (2006): Scanning probe microscopy of plant cell wall and its constituents: In: Force microscopy. Applications in biology and medicine. (Eds. B. Jena, H. Hörber), Wiley, pp 95-113.

Kalauzi A., Mutavdžić D., Djikanović D., Radotić K., Jeremić  M. (2007): Application of Asymmetric Model in Analysis of Fluorescence Spectra of Biologically Important Molecules. J. Fluorescence 17  319-329.

Radotić K., Djikanović D., Bogdanović J., Vasiljević-Radović D. (2008) Levels of plant cell wall structural organization revealed by atomic force microscopy. Journal of Microscopy 232, 508–510.

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Education/Professional Career

1986:

B.Sc. Molecular Biology and Physiology, University of Belgrade

1990:

M.Sc. Biophysics, University of Belgrade

1994:

D.Sc. Biology (Plant biophysics and biochemistry), University of Belgrade

present position:

Leading Scientific Associate, Department of life sciences, Institute for Multidisciplinary Research, Belgrade

Others

Society membership:

  • International polyphenol group
  • European society for microscopy